Sieve-ID Diagnostics for Medical

Sepsis in blood

Blood poisoning, or sepsis, is a severe and sometimes fatal disease that is often caused by a bacterial infection or their products. Rapid and early treatment of a patient is essential to limit lethal or permanent effects. The death rate by sepsis is calculated to be 30% - 40% in countries with modern healthcare and intensive care facilities.

Analysis of blood samples for sepsis takes 24 hrs up to 48hrs. In case of presence of a micro-organism a microscopy sample is prepared and the cells are grown on media. These tests, essential to determine the organism species and antibiotic resistance take about two more days.

To reduce the required time for analysis, several tests have successfully been developed that are able to identify the micro-organism from a positive blood sample enrichment (24hr-48hr incubation).

Advantage of these tests is that the micro-organism is identified (including potential antibiotic resistance) within a few hours. However, a blood sample incubation remains obligatory due to the low amount of bacterial cells present in a blood sample.

Recently a Nano4Vitality project was started in which Innosieve Diagnostics BV will develop a Sieve-ID detection kit for the direct detection of bacterial cells in blood samples. The main focus is to omit incubation of the blood sample. This will provide a rapid screening of a patient's infection. Herewith a personal treatment can be started, essentially to increase a succesful recovery of the patient.

Progress of the N4V project can be found here soon.

Pathogen quantification in urine

In current urine diagnostics, detection is often based on nitrate reduction in a sample. The level of nitride is measured in a urine sample, indicating the presence of microbial cells in urine. However, these tests are not sensitive (threshold of 10e5 cells, less in not detected) and have a high incidence of false negative results; 15% of the causal agents of urinatory tract infections, do not reduce nitrated in nitride and is thus not identified positive.

In a current national funded project called NanonextNL, Innosieve will develop a rapid assay for simultaneous detection and quantification of four groups of pathogens related to urinatory tract infections, namely Staphylococcus aureus, Escherichia coli, Enterococcus and Pseudomonas aeruginosa.

Progress of the NanonextNL project can be found here soon.

Back to Products page